Endodontic Practice Cleaning and Sanitization Protocol

1. Treatment Room Sanitization

Between Patients

• Remove all disposable barriers
• Clean all surfaces with EPA-registered hospital-grade disinfectant
• Special attention to:
  • Dental chair and controls
  • Microscope handles and controls
  • X-ray equipment
  • Computer keyboards (if not barrier-protected)

End of Day

• Deep clean all surfaces
• Flush dental unit water lines with approved antimicrobial solution
• Clean and disinfect floors
• Empty all waste containers

2. Instrument Processing

Pre-sterilization

1. Wear appropriate PPE
2. Remove visible debris
3. Ultrasonic cleaning with enzymatic solution
4. Manual cleaning of instruments with special attention to:
   • Endodontic files
   • Rotary instruments
   • Hand instruments

Sterilization

• Package instruments in FDA-approved sterilization pouches
• Use Class 5 integrators in each load
• Document sterilization cycles
• Maintain sterilizer logs

3. Special Considerations

Microscope Care

• Clean objective lens with approved optical cleaner
• Disinfect handles and controls
• Replace disposable barriers between patients

CBCT Equipment

• Clean chin rest and positioning devices
• Disinfect control panel
• Weekly deep cleaning of equipment surfaces

4. Documentation Requirements

• Daily cleaning logs
• Sterilization records
• Water line testing results
• Equipment maintenance records

5. Staff Responsibilities

Clinical Staff

• Room turnover between patients
• Instrument processing
• Equipment cleaning

Support Staff

• End of day cleaning
• Supply restocking
• Documentation maintenance

6. Quality Assurance

• Weekly spore testing
• Monthly water quality testing
• Quarterly protocol review
• Annual staff training